Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptidein human plasma
نویسندگان
چکیده
منابع مشابه
Immunoradiometric assay of atrial natriuretic peptide in unextracted plasma.
We have developed and validated a two-site liquid-phase immunoradiometric assay (IRMA) of atrial natriuretic peptide (ANP) in unextracted human plasma. Both radiolabeled rabbit anti-ANP IgG and polyclonal mouse anti-ANP must bind to ANP for detection, and the assay is specific for peptides with both an intact C-terminus and a disulfide bridge. The assay sensitivity (detection limit) is 0.96 pmo...
متن کاملPlasma N-terminal proatrial natriuretic peptide concentration in cats with hypertrophic cardiomyopathy
... ... ii Acknowledgements . .. . iv Table of Contents .. . v List of Figures . . .. vi List of Abbreviations ... .. vii Introduction . ... 1 Chapter I: Literature Review . .... 4 I. Atrial Natriuretic Peptide .. 4 A. Introduction . 4 B. ANP ...
متن کاملPlasma brain natriuretic peptide and N-terminal proatrial natriuretic peptide levels in obese patients: a cause or result of hypertension?
Proatrial Natriuretic Peptide Levels in Obese Patients: A Cause or Result of Hypertension? To the Editor: We read with great interest the paper by Wang et al1 indicating that obese subjects had lower brain natriuretic peptide (BNP) and N-terminal proatrial natriuretic peptide (N-terminal proANP) levels than did lean subjects in a large sample population of the Framingham Heart Study. They specu...
متن کاملEvaluation of the association between plasma concentration of N-terminal proatrial natriuretic peptide and outcome in cats with cardiomyopathy.
OBJECTIVE To determine whether plasma N-terminal proatrial natriuretic peptide (NT-proANP) concentration could predict the outcome (survival duration) of cats with cardiomyopathy (CM). DESIGN Case-control study. ANIMALS 51 cats with CM (25 with and 26 without congestive heart failure [CHF]) and 17 healthy cats. PROCEDURES Cats were thoroughly examined and assigned to 1 of 3 groups (contro...
متن کاملImproved immunoradiometric assay for plasma renin.
BACKGROUND Our renin IRMA overestimated renin in plasmas with high prorenin-to-renin ratios. We suspected that the overestimation of renin was caused less by cross-reactivity of the renin-specific antibody with prorenin than by a conformational change of prorenin into an enzymatically active form during the assay. METHODS Because the inactive form of prorenin converts slowly into an active fo...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1998
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/44.5.1008